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TAPSO Biological Buffer N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxy propane sulfonic acid
Quick detail:
TAPSO Biological Buffer N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropansulfonic acid CAS NO 68399-81-5 99%
| Name | TAPSO | Appearance | White crystalline powder |
| Assay,% | ≥99.0 | Loss on drying,% | ≤0.5 |
| PH(1% aq.) | 4.7-5.3 | Solubility(10% aq.) | Clear, colorless solution |
| Heavy metals,ppm | ≤5 | Ultraviolet absorbance/280nm(10% aq.) |
|
Description:
> Formula:C7H17NO7S
> Weight: 259.277
> Storage:room temperature
> Safety Description:S24/25
> Danger Description:R36/37/38
> Danger Symbol: Xi
1.Applications:
A.A test kit for detection of high-density lipoprotein cholesterol
B.Method and buffering system for continuous stabilization of pH of cell, gamete and embryo
culture media
2.Location:
A.biology/biochemistry laboratory, pharmaceutical factory, cosmetics factory,dyestuff factory
Synonyms:
1-propanesulfonicacid,2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]ami;TAPSO;
N-(TRIS(HYDROXYMETHYL)METHYL)-3-AMINO-2-HYDROXYPROPANE SULFonIC ACID;
2-Hydroxy-N-(tris(hydroxymethyl)methyl)-3-aminopropanesulfonic acid;
3-[N-TRIS(HYDROXYMETHYL)METHYLAMINO]-2-HYDROXYPROPANESULFonIC ACID;
3-[N-TRIS-(HYDROXYMETHYL)METHYLAMINO]-2-HYDROXYPROPANESULPHonIC ACID;
2-Hydroxy-3-[tris(hydroxymethyl)methylamino]-1-propanesulfonic acid ;
2-hydroxy-3-[[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]amino]propanesulphonic acid;
N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxypropanesulfonic acid;
3-{[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}-2-hydroxypropane-1-sulfonic acid;
(2R)-2-hydroxy-3-{[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]ammonio}propane-1-sulfonate;
(2S)-2-hydroxy-3-{[2-hydroxy-1,1-bis(hydroxymethyl)ethyl]ammonio}propane-1-sulfonate;
N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydro-xypropanesulfonic acid
Related categories:Biochemistry;Good's Buffers;Buffer
Related Products:7365-44-8;68189-43-5;29915-38-6
The determination of PH
Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, determination of the pH used have been calibrated pH meter.
The determination of solubility
Weigh a certain amount of samples into test tube, add a certain amount of water, shake and dissolve, observe the solution.
Detection of heavy metals
The meaning of heavy metals of this act is under the specified experimental condition, which can coloration effects with sodium sulfide or thioacetamide .The glasses of preparation or storage is not allowed to contain lead.
5.8.1 Reagents
a) Lead dinitrate:1μg/mL
b) Sodium hydroxide :10%(m/v)
c) Sodium sulfide :10%(m/v)
5.8.2 Preparation of lead standard solution
Weigh lead dinitrate 0.1599g ,relocated to 1000mL volumetric flask, plus nitric acid 5mL and water 50mL, diluted with water to the mark , shake, as the stock solution.
5.8.3 Determination of step
Before use, the exact amount of stock solution 1mL, relocated to a 100ml volumetric flask, dilute with water to the mark, shake, limit the use of the day .
Weigh sample 1g, after adding sodium hydroxide solution 5mL and 20mL of water to dissolve, set Nessler colorimetric tube, add 5 drops of
TAPSO Biological Buffers N-[Tris(hydroxymethyl)methyl]-3-amino-2-hydroxy propane sulfonic acid
